determination of the unbound clozapine concentration in human serum albumin ( has ), human plasma, rabbit serum and plasma samples by capillary electrophoresis in the frontal analysis mode ( ce / fa ) was developed 摘要采用毛細(xì)管電泳-迎頭分析模式體外實(shí)驗(yàn)測定了人血清白蛋白溶液、人血漿、兔血清和血漿樣品溶汲中游離氯氮平的濃度。
cercariae were collected, cultured in vitro and transformed to schistosomula in the rpmi 1640 medium with rabbit serum . the schistosomula were cultured in conditional media up to 96 hours . the number of schistosomula was counted and the death rate was calculated 將條件培養(yǎng)基與童蟲共培養(yǎng),96h內(nèi)連續(xù)觀察計數(shù),計算童蟲死亡率,與陰性對照比較,童蟲死亡率最高的基因池進(jìn)入下一輪篩選。
serum samples have been assayed and the results demonstrate feasibility of the proposed immunosensor for clinical analysis . ( 2 ) in chapter 2, a renewable amperometric immunosensor has been proposed for the determination of schistosoma-japonium antibody ( sjab ) in rabbit serum . the experimental conditions of sjab determination were studied and optimized 結(jié)果與臨床診斷用的elisa方法相比較,令人滿意八2)在第二章中報導(dǎo)了制備可重復(fù)使用的日本血吸蟲電化學(xué)免疫傳感器,對r本血吸蟲抗體(sjab)的實(shí)驗(yàn)條件進(jìn)行了研究和優(yōu)化。
the target protein purified by ni-nta metal chelate affinity presented one major band in the sds-page and reacted with rabbit serum raised against the ndv b95 strain in western blotting, suggested that the protein could be used as one of candidated antigenes . it provided the basis for further studying on recombinant vaccine against ndv 經(jīng)westem-bloting雜交實(shí)驗(yàn),純化出目的蛋白與兔抗ndvb95株抗體反應(yīng),說明表達(dá)產(chǎn)物確為ndvb95株hn蛋白,且具有一定的免疫原性,為進(jìn)一步研究重組疫苗創(chuàng)造了一定的條件。